Lab 3: Cartilage & Bone
Unfortunately, these images have taken on a more basophilic hue online then they actually are, because as you know, bone is acidophilic. Why?
Identify cells A, B & C. Why is cell A basophilic? What accounts for the basophilia and the acidophilia in cell C?
Osteoblasts (A) are basophilic due to rough endoplasmic reticulum. Remember, they are producing the collagen that makes the bone stain acidophilic. When osteoblasts become surrounded by the matrix they are producing, they then become osteocytes (B). Osteoclasts (C) also contain rough endoplasmic reticulum to produce the lysosomes that are released to dissolve bone. The lysosomes account for the acidophilia in these cells.